Adipogenic constituents from the bark of Larix laricina du Roi (K. Koch; Pinaceae), an important medicinal plant used traditionally by the Cree of Eeyou Istchee (Quebec, Canada) for the treatment of type 2 diabetes symptoms

作者：Shang Nan; Guerrero Analco Jose A; Musallam Lina; Saleem Ammar; Muhammad Asim; Walshe Roussel Brendan; Cuerrier Alain; Arnason John T^*; Haddad Pierre S

来源：Journal of Ethnopharmacology, 2012, 141(3): 1051-1057.

DOI：10.1016/j.jep.2012.04.002

• 3T3-L1 adipocytes
• Adipogenesis
• Bioassay-guided fractionation
• Tamarack

Ethnopharmacological relevance: Diabetes is a growing epidemic worldwide, especially among indigenous populations. Larix laricina was identified through an ethnobotanical survey as a traditional medicine used by Healers and Elders of the Cree of Eeyou Istchee of northern Quebec to treat symptoms of diabetes and subsequent in vitro screening confirmed its potential. %26lt;br%26gt;Materials and methods: We used a bioassay-guided fractionation approach to isolate the active principles responsible for the adipogenic activity of the organic extract (80% Et0H) of the bark of Larix laricina. Post-confluent 3T3-L1 cells were differentiated in the presence or absence of the crude extract, fractions or isolates of Larix laricina for 7 days, then triglycerides content was measured using AdipoRed reagent. %26lt;br%26gt;Results: We identified a new cycloartane triterpene (compound 1), which strongly enhanced adipogenesis in 3T3-L1 cells with an EC50 of 7.7 mu M. It is responsible for two thirds of the activity of the active fraction of Larix laricina. The structure of compound 1 was established on the basis of spectroscopic methods (IR, HREIMS, 1D and 2D NMR) as 23-oxo-3 alpha-hydroxycycloart-24-en-26-oic acid. We also identified several known compounds, including three labdane-type diterpenes (compounds 2-4), two tetrahydrofuran-type lignans (compounds 5-6), three stilbenes (compounds 7-9), and taxifolin (compound 10). Compound 2 (13-epitorulosol) also potentiated adipogenesis (EC50 8.2 mu M) and this is the first report of a biological activity for this compound. %26lt;br%26gt;Conclusions: This is the first report of putative antidiabetic principles isolated from Larix laricina, therefore increasing the interest in medicinal plants from the Cree pharmacopeia.

• 出版日期2012-6-14