Individual and combined effects of mesenchymal stromal cells and recombinant stimulatory cytokines on the in vitro growth of primitive hematopoietic cells from human umbilical cord blood

作者：Flores Guzman Patricia; Flores Figueroa Eugenia; Montesinos Juan J; Martinez Jaramillo Guadalupe; Fernandez Sanchez Veronica; Valencia Plata Ignacio; Alarcon Santos Guadalupe; Mayani Hector^*

来源：Cytotherapy, 2009, 11(7): 886-896.

DOI：10.3109/14653240903180076

• cell expansion
• cell proliferation
• cytokines
• hematopoietic progenitors
• in vitro
• mesenchymal stromal cells

Background aims We have previously characterized the in vitro growth of two cord blood-derived hematopoietic cell populations in liquid cultures supplemented with recombinant cytokines. In the present study, we assessed the effects of bone marrow-derived mesenchymal stromal cells (MSC) on the growth of such cells.
Methods CD34( ) CD38( ) Lin(-) and CD34( ) CD38(-) Lin(-) cells were obtained by negative selection, and cultured in the presence of marrow-derived MSC and/or early-and late-acting cytokines. Hematopoietic cell growth was assessed throughout a 30-day culture period.
Results In the presence of MSC alone, both populations showed significant proliferation. Direct contact between MSC and CD34( ) cells was fundamental for optimal growth, especially for CD34( ) CD38(-) Lin(-) cells. In the presence of early-acting cytokines alone, cell growth was significantly higher than in cultures established with MSC but no cytokines. In cultures containing both MSC and early-acting cytokines, a further stimulation was observed only for CD34( ) CD38(-) Lin(-) cells. The cytokine cocktail containing both early-and late-acting cytokines was significantly more potent at inducing hematopoietic cell growth than the early-acting cytokine cocktail. When cultures were supplemented with early-and late-acting cytokines, MSC had no further effect on the growth of hematopoietic cells.
Conclusions MSC seem to play a key role, particularly on more primitive (CD34( ) CD38(-) Lin(-)) cells, only in the absence of cytokines or the presence of early-acting cytokines. When both early-and late-acting cytokines are present in culture, MSC seem to be unnecessary for optimal development of CFC and CD34( ) cells.

• 出版日期2009