DETERMINATION OF ZEARALENONE BY THE CAPILLARY ZONE ELECTROPHORESIS-UV DETECTION AND ITS APPLICATION TO POULTRY FEED AND CEREALS

作者：Guray Tufan^*; Tuncel Muzaffer; Uysal Ulku Dilek; Oncu Kaya Elif Mine

来源：Journal of Liquid Chromatography & Related Technologies, 2013, 36(10): 1366-1378.

DOI：10.1080/10826076.2012.691437

• capillary electrophoresis
• grain
• maize flour
• mycotoxin
• poultry feed
• zearalenone

A capillary zone electrophoretic method for the determination of zearalenone is described in this study. In the separation, a run buffer consisting of 20mM sodium tetraborate at pH 9.0 with 15% acetonitrile applying 15kV, injecting 10s at 50 mbar was utilized. Phenobarbital was a suitable internal standard and the signals were recorded at 254nm. Zearalanon and internal standard appeared at 5.46min (RSD% 0.20) and 6.35min (RSD% 0.18), respectively. The repeatability results are in the range of 0.011.58 for inter-day. The linearity was investigated in the range of 5.20x107M to 7.86x105M ZEA and it was linear fitting to the equation of [rPN=244803.8 C(M) 0.0348; r=0.9999]. The LOD and LOQ were calculated to be 2.70x108M (8.25 mu g/L) and 8.17x108M (25 mu g/L), respectively. Then, the method was successfully applied to the analysis of ZEA in poultry feeds, flour of maize, grain, and certain cereal samples such as fibrous biscuit, popcorn, and rice crisp.

• 出版日期2013-4-1