Development of anti-CD47 single-chain variable fragment targeted magnetic nanoparticles for treatment of human bladder cancer

作者：Rezaei Gashin; Habibi Anbouhi Mahdi^*; Mahmoudi Morteza; Azadmanesh Kayhan; Moradi Kalbolandi Shima; Behdani Mahdi; Ghazizadeh Leila; Abolhassani Mohsen^*; Shokrgozar Mohammad Ali^*

来源：Nanomedicine, 2017, 12(6): 597-613.

DOI：10.2217/nnm-2016-0302

• bladder cancer cell line
• CD47
• magnetic nanoparticle
• phage display
• scFv
• thermotherapy
• tumor cell detection

Aim: To develop a novel anti-CD47 single-chain variable fragment (scFv) functionalized magnetic nanoparticles (MNPs) for targeting bladder cell lines and its applicability in thermotherapy. Material & methods: An immunized murine antibody phage display library was constructed and screened to isolate anti-CD47 binders. A scFv was selected and conjugated to MNPs which was then utilized to discriminate CD47(+) bladder cells along with assessing its efficacy in thermotherapy. Results: An scFv with high affinity to bladder cells was efficiently conjugated to MNPs. Following a hyperthermia treatment, the function of scFv-MNP conjugates led to a considerable reduction in cell viability. Conclusion: The anti-CD47 scFv-MNP conjugate was an effective cancer cell thermotherapy tool that might pave the way for development of bionano-based targeting techniques in both early detection and treatment of cancer.

• 出版日期2017-3