Preparation of biomolecule gel matrices for electron microscopy

作者：Padalkar Sonal; Zhao Jianxiu; Stuart Kate; Panitch Alyssa; Rickus Jenna; Stanciu Lia^*

来源：Ultramicroscopy, 2008, 108(4): 309-313.

DOI：10.1016/j.ultramic.2007.04.004

• electron microscopy
• gels
• biomolecules

We report a new sample preparation method that allows the direct transmission electron microscopy evaluation of the architectural characteristics of biomolecules entrapped in gel matrices. We demonstrate that this sample preparation technique can be used for the identification and ultrastructural characterization of liposomes, collagen I and collagen III embedded in gel matrices, and has the potential to be useful for transmission electron microscopy (TEM) characterization of other biornolecule-gel matrix systems.

• 出版日期2008-3