Ascorbate promotes carbon tetrachloride-induced hepatic injury in senescence marker protein 30-deficient mice by enhancing inflammation

作者：Ki Mi Ran; Lee Hye Rim; Park Jin Kyu; Hong Il Hwa; Han Seon Young; You Sang Young; Lee Eun Mi; Kim Ah Young; Lee Seung Sook; Jeong Kyu Shik^*

来源：Journal of Nutritional Biochemistry, 2011, 22(6): 535-542.

DOI：10.1016/j.jnutbio.2010.04.008

• Ascorbate-deficient animal
• Inflammation
• Liver injury
• Pro-oxidant
• Senescence
• SMP30

The genetic deletion of the senescence marker protein 30 (SMP30) gene results in ascorbate deficiency and the premature aging processes in mice. Apparent liver injury of SMP30(-/-) mice was less severe than those of wild type (WT) mice, upon chronic CCl(4) injection. The purpose of this study was to investigate the pathophysiology underlying the mild CCl(4) toxicity in SMP30(-/-) mice. Along with the lower level of serum alanine aminotransferase, the livers of SMP30(-/-) mice revealed a lesser glycogen depletion, a decrease in c-Jun N-terminal kinase (JNK)-mediated inflammatory signaling in parallel with tumor necrosis factor-alpha and interleukin-1 beta, inducible nitric oxide synthase and glutathione peroxidase, and the lower lipid peroxidation as compared to those of WT mice. CCl(4)-induced proliferation, measured by the expression of proliferating cell nuclear antigen, was low in SMP30(-/-) mice as compared with that of WT mice whereas the levels of p21 and Bax were comparable to those of the CCl(4)-treated WT mice. Moreover, CCl(4) toxicity in ascorbate-fed SMP30(-/-) mice was comparable to that of the CCl(4)-alone treated WT mice, accompanied by an increase in the above mentioned factors. Conversely, ascorbate partly compensated for the CCl(4)-induced oxidative stress in WT mice, indicating that sufficient ascorbate may be required for an antioxidant function under severe levels of oxidative stress. Our data suggest that the restoration of ascorbate-deficiency reverses a sluggish immune system into an activated condition by an increase in JNK-mediated inflammation and free radical cascade; thus leading to accelerated hepatic damage in SMP30(-/-) mice.

• 出版日期2011-6