Development and Evaluation of Tri-Functional Immunoliposomes for the Treatment of HER2 Positive Breast Cancer

作者：Vaidya Tanaya; Straubinger Robert M; Ait Oudhia Sihem^*

来源：Pharmaceutical Research, 2018, 35(5): UNSP 95.

DOI：10.1007/s11095-018-2365-x

• anti-CD3
• HER2-positive breast cancer
• immunoliposomes
• trastuzumab
• doxorubicin

Purpose Trastuzumab combined with Doxorubicin (DOX) demonstrates significant clinical activity in human epidermal growth factor receptor-2 (HER2)-positive breast cancer (BC). However, emergence of treatment resistance and trastuzumab associated cardiotoxicity remain clinical challenges. In an effort to improve patient outcome, we have developed and evaluated novel tri-functional immunoliposomes (TFIL) that target HER2-receptors on BC cells and CD3-receptors on Tlymphocytes, and deliver DOX.
Methods Trastuzumab (anti-HER2) and OKT-3 (anti-CD3) antibodies were conjugated to liposomes using a micelletransfer method. Cytotoxicity of targeted immunoliposomes loaded withDOXwas examined in vitro onHER2-positive BC cells (BT474), with peripheral blood monocytic cells (PBMC) as immune effector cells.
Results TFIL demonstrated high antibody-liposome conjugation ratios (100-130 mu g protein/mu mol phospholipid) and cargo capacity (0.21 mol: mol drug: lipid), highly efficient DOX loading (> 90%), a particle size favorable for extended circulation (similar to 150 nm), and good stability (up to 3 months at 4 degrees C). In the presence of PBMCs, TFIL showed complete killing of BT474 cells, and were superior to mono-targeted trastuzumab-bearing liposomes, non-targeted liposomes, and free Trastuzumab and DOX.
Conclusions Novel anti-HER2xCD3 + DOX TFIL show promise as a means to both engage immune cells against HER2 positive breast cancer cells and deliver chemotherapy, and have the potential to improve clinical outcomes.

• 出版日期2018-5