A distinctive set of genes is upregulated during the inflammation-carcinoma sequence in mouse stomach infected by helicobacter felis

作者：Kobayashi Motohiro; Lee Heeseob; Schaffer Lana; Gilmartin Tim J; Head Steven R; Takaishi Shigeo; Wang Timothy C; Nakayama Jun; Fukuda Minoru^*

来源：Journal of Histochemistry and Cytochemistry, 2007, 55(3): 263-274.

DOI：10.1369/jhc.6A7097.2006

• Helicobacter felis
• insulin-gastrin transgenic mouse
• high endothelial venule-like vessel
• peripheral lymph node addressin
• inflammation-carcinoma sequence
• carcinogenesis
• surfactant protein D
• CD74

Helicobacter pylori infects over half the population worldwide and is a leading cause of chronic gastritis and gastric cancer. However, the mechanism by which this organism induces inflammation and carcinogenesis is not fully understood. In the present study we used insulin-gastrin (INS-GAS) transgenic mice that fully develop gastric adenocarcinoma after infection of H. pylori-related Helicobacter felis. Histological examination revealed that more than half of those mice developed invasive adenocarcinoma after 8 months of infection. These carcinomas were stained by NCC-ST-439 and HECA-452 that recognize 6-sulfated and non-sulfated sialyl Lewis X. Lymphocytic infiltration predominantly to submucosa was observed in most H. felis-infected mice, and this was associated with the formation of peripheral lymph node addressin (PNAd) on high endothelial venule (HEV)-like vessels detected by MECA-79. Time-course analysis of gene expression by using gene microarray revealed upregulation of several inflammation-associated genes including chemokines, adhesion molecules, surfactant protein D (SP-D), and CD74 in the infected stomach. Immunohistochemical analysis demonstrated that SP-D is expressed in hyperplasia and adenocarcinoma whereas CD74 is expressed in adenocarcinoma in situ and invasive carcinoma. These results as a whole indicate that H. felis induces HEV-like vessels and inflammation-associated chemokines and chemokine receptors, followed by adenocarcinoma formation.

• 出版日期2007-3
• 单位河北医科大学