Alterations in the Activity and Structure of Pectin Methylesterase Treated by High Pressure Carbon Dioxide

作者：Zhou Linyan; Wu Jihong; Hu Xiaosong; Zhi Xian; Liao Xiaojun^*

来源：Journal of Agricultural and Food Chemistry, 2009, 57(5): 1890-1895.

DOI：10.1021/jf803501q

• Pectin methylesterase
• high pressure carbon dioxide
• inactivation
• structure

The influence of high pressure carbon dioxide (HPCD) on the activity and structure of pectin methylesterase (PME) from orange was investigated. The pressures were 8-30 MPa, temperature 55 degrees C and time 10 min. HPCD caused significant inactivation on PME, the lowest residual activity was about 9.3% at 30 MPa. The SDS-PAGE electrophoretic behavior of HPCD-treated PME was not altered, while changes in the secondary and tertiary structures were found. The beta-structure fraction in the secondary structure decreased and the fluorescence intensity increased as HPCD pressures were elevated. After 7-day storage at 4 degrees C, no alteration of its activity and no reversion of its,beta-structure fraction were observed, while its fluorescence intensity further decreased.

• 出版日期2009-3-11
• 单位中国农业大学