Alteration of Seed Storage Protein Composition in Soybean [Glycine max (L.) Merrill] Mutant Lines Induced by gamma-Irradiation Mutagenesis

作者：Lee Kyung Jun; Kim Jin Baek; Kim Sang Hoon; Ha Bo Keun; Lee Byung Moo; Kang Si Yong; Kim Dong Sub^*

来源：Journal of Agricultural and Food Chemistry, 2011, 59(23): 12405-12410.

DOI：10.1021/jf202809j

• gamma ray
• mutation
• SDS-PAGE
• seed storage protein
• soybean

This study investigated the alteration of seed storage proteins in soybean mutants induced by gamma-irradiation. Five soybean cultivars and four landraces were irradiated with 250 Gy of gamma rays to induce variability. The seed storage protein profiles of 414 genetic fixed mutants (M(12)-M(20)) having excellent agricultural traits were analyzed by SDS-PAGE. Among the 414 mutants, 58 were identifed as lacking lipoxygenase, 89 lacking the alpha' subunit, 113 lacking the alpha subunit, and 40 with an altered beta subunit. One hundred and forty-nine mutants lacked the A(3) subunit of glycinin. Fifty-four mutants showed higher trypsin inhibitor (TIA) activity, whereas 139 showed lower TIA activity compared to their original cultivars. The selected mutants with low amounts of antinutritional factors such as trypsin inhibitor, lipoxygenase, and alpha subunit will constitute genetic resources for improving soybean protein quality.

• 出版日期2011-12-14