A highly sensitive and direct competitive enzyme-linked immunosorbent assay for the detection of di-(2-ethylhexyl) phthalate (DEHP) in infant supplies

作者：Zhang, Mingcui^*; Hong, Wentong; Wu, Xiayu; Zhang, Yue; Li, Fengzhu; Zhao, Su-Qing

来源：Analytical Methods, 2015, 7(13): 5441-5446.

DOI：10.1039/c5ay00207a

• SOLID-PHASE MICROEXTRACTION
• DIETHYL PHTHALATE
• FLUORESCENCE IMMUNOASSAY
• REPRODUCTIVE TOXICITY
• ESTERS
• BIODEGRADATION
• EXTRACTION
• PRODUCTS
• ELISA
• ACID

A sensitive and specific direct competitive enzyme-linked immunosorbent assay (dc-ELISA) was studied in this paper for the detection of di-(2-ethylhexyl) phthalate (DEHP) based on an antigen-coating format. The DEHP-specific polyclonal antibody was raised in rabbits and used to construct the dc-ELISA for the measurement of DEHP. The conjugates of the antibody with horseradish peroxidase (HRP) were used as the detection probe. Under optimal conditions, the assay had a detection limit (LOD) of about 0.0042 ng mL(-1), with an apparent linear range of 10(-3) - 10(3) ng mL(-1) (R-2 = 0.998). The cross-reactivity with six other structurally related phthalate esters was below 1%. The recoveries of DEHP ranged from 80.8% to 119.2% indicating that the method was successfully applied for the determination of DEHP in infant supplies.

• 出版日期2015
• 单位广东工业大学; 安徽师范大学