Application of iTRAQ to screen differentially expressed proteins in the hippocampus of a mouse model of Down syndrome

作者：Yu, Bin; Zhang, Bin; Zhou, Wenbo; Kong, Jing; Yuan, Pei; Jiang, Jian^*; Wang, Qiuwei^*

来源：International Journal of Clinical and Experimental Pathology, 2016, 9(10): 10483-+.

• Down syndrome
• proteomics
• iTRAQ
• Ts65Dn
• hippocampus

Background: Down syndrome (DS) is characterized by mental retardation and the development of Alzheimer's disease (AD). However, the reason was not yet clear. Methods: Ts65Dn mice were used in this study. We collected their hippocampi and identified protein biomarkers using iTRAQ. We used bioinformatics to analyze differentially expressed proteins and western blots to preliminarily verify iTRAQ results. Results: A total of 2805 proteins were identified in the hippocampus of mouse. After calculating the index of significance (p-Value) and statistically analyzing differences between the DS and control groups, we found 374 significant differentially expressed proteins. These included protein binding, proteins with catalytic activity, and proteins with transferase activity. In addition, they were related to cellular processes, regulation of biological processes, and metabolic processes. The top 7 differentially regulated proteins were uncharacterized protein C2 orf 47 homolog (mitochondrial), isoform 2 of filamin A-interacting protein 1-like, zinc finger protein, isoform 1 of pericentriolar material 1 protein, SEC23-interacting protein, BAG family molecular chaperone regulator 3, and serpin H1. Conclusions: We observed differentially expressed proteins in the hippocampi of Ts65Dn mice that may be closely related to their neurological deficits. The new generation iTRAQ technology helps in the screening and identification of these proteins.

• 出版日期2016
• 单位南京医科大学