Avian metapneumovirus M2:2 protein inhibits replication in Vero cells: Modification facilitates live vaccine development

作者：Clubbe Jayne; Naylor Clive J^*

来源：Vaccine, 2011, 29(51): 9493-9498.

DOI：10.1016/j.vaccine.2011.10.024

• Metapneumovirus
• Reverse genetics
• Vaccine
• Vero replication

Throughout the world, avian metapneumovirus (AMPV) infection of subtype A is principally controlled by two live vaccines both derived from UK field strain #8544. Improvements of those vaccines by use of reverse genetics technology was found to be hampered by the inability of #8544 to replicate in the commonly exploited Vero cell based reverse genetics system. A systematic reverse genetics based genome modification of a DNA copy of #8544, employing sequence data from a Vero grown, #8544 derived, live vaccine; was used to determine mutations required to facilitate virus recovery and replication in Vero cells. This identified a single coding substitution in the M2:2 reading frame as responsible. Furthermore, ablation of M2:2 was found to elicit the same outcome. M2:2 sequence analysis of seven AMPVs found Vero cell adaption to be associated with non similar amino acid changes in M2:2. The study shows that M2:2 modification of field virus #8544 will enable research leading to improved vaccines. This may have more general application to other AMPV field strains.

• 出版日期2011-11-28