Mannosylated structures of mycobacterial lipoarabinomannans facilitate the maturation and activation of dendritic cells

作者：Zhang, Shaopeng; Wu, Qihang; Lei, Hang; Zheng, Hui; Zhou, Fang; Sun, Zhanqiang; Zhao, Junwei; Yu, Xiaoli^*; Zhang, Shulin^*

来源：Cellular Immunology, 2019, 335: 85-92.

DOI：10.1016/j.cellimm.2018.11.007

• Mycobacterium tuberculosis
• Lipoarabinomannan
• Dendritic Cell
• Cytokine

Lipoarabinomannan (LAM) is an important virulent factor secreted by mycobacteria, which generally elicit a strong immune response in the host. In this study, the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc(2)155 and a newly discovered clinical isolate, M. sp. QGD101, was analyzed and further evaluated whether these LAMs can induce DC maturation and promote the immunomodulatory properties. The results reveal that the major structural difference of these LAMs is the amount of mannosyl residues, especially at the terminal end of LAM, which play a key role in determining the divergent response of DCs after mycobacterial infection. Also, this study indicates an important relevance between the glycosylated structure of LAM and its immunomodulatory property, which is helpful to develop a potential approach for identification of different mycobacteria and also lays a foundation for the development of a novel polysaccharide immunological strategy against tuberculosis.

• 出版日期2019-1
• 单位上海交通大学; 武汉轻工大学; 郑州大学