Development of the fingerprint for the quality of Radix Linderae through ultra-pressure liquid chromatography-photodiode array detection/electrospray ionization mass spectrometry

作者：Wu, Yong-jiang; Zheng, Yun-liang; Luan, Lian-jun; Liu, Xue-song^*; Han, Zheng; Ren, Yi-ping; Gan, Li-she; Zhou, Chang-xin

来源：Journal of Separation Science, 2010, 33(17-18): 2734-2742.

DOI：10.1002/jssc.201000243

• Fingerprint analysis
• Quality control
• Radix Linderae
• Sesquiterpene lactones
• Ultra-pressure LC-photodiode array detector-MS

An ultra-pressure LC (UPLC) method has been developed and validated for the quality evaluation of a traditional Chinese medicine (Radix Linderae) by chemical fingerprint analysis with chromatograms collected at two wavelengths (260 and 320 nm). Eleven characteristic peaks in the fingerprints were identified by comparing their retention times, UV spectra and ESI-MS/MS data with those of the reference substances or the data in the literatures. Both correlation coefficient of similarities in chromatograms and relative peak areas of common peaks were calculated for quality expression of the Radix Linderae samples collected from different areas in China. The results showed high variation of relative peak area and correlation coefficients among the samples collected from various habitats, which indicated that the quality consistency of Radix Linderae is still a problem worthy of serious concern.

• 出版日期2010-9
• 单位浙江省疾病预防控制中心; 浙江大学