Zhuanggu Jianxi Decoction (a pound (R) e(a)ayene dagger eae-(1)) limits interleukin-1 beta-induced degeneration chondrocytes via the caveolin-p38 MAPK signal pathway

作者：Yan Hu; Su You xin^*; Lin Xue yi; Chen Bao jun; Zhang Qing; Zhang Zi yi; Wang Yi ru; Li Ya nan; Lu Mei li; He Zhen; Sheng Lu; Wang Wen ting

来源：Chinese Journal of Integrative Medicine, 2014, 20(5): 353-359.

DOI：10.1007/s11655-014-1801-y

• osteoarthritis
• Zhuanggu Jianxi Decoction
• chondrocyte degeneration
• caveolin-p38 pathway
• serum pharmacology

To evaluate the effect of Zhuanggu Jianxi Decoction (a pound (R) e(a)ayene dagger eae-(1), ZGJXD) on interleukin-1 beta (IL-1 beta)-induced degeneration of chondrocytes (CDs) as well as the activation of caveolin-p38 mitogen-activated protein kinase (MAPK) signal pathway, investigating the possible molecular mechanism that ZGJXD treats osteoarthritis.
Serum pharmacology was applied in the present study, where ZGJXD was orally administrated to New Zealand rabbits and then ZGJXD containing serum (ZGJXD-S) was collected for following in vitro experiments. CDs were isolated aseptically from New Zealand rabbits and then cultured in vitro. Upon IL-1 beta stimulation, the degeneration of CDs was verified by inverted microscope, toluidine blue stain and type II collagen immunocytochemistry. After IL-1 beta-stimulated CDs were intervened with blank control serum, ZGJXD-S, together with or without SB203580 (a specific inhibitor of p38 MAPK) for 48 h, caveolin-1 protein expression and the phosphorylation level of p38 were determined by Western blotting, and the mRNA expression of IL-1 beta, tumor necrosis factor alpha (TNF-alpha), matrix metalloproteinase 3 (MMP-3) and MMP-13 were examined by real-time polymerase chain reaction.
IL-1 beta stimulation induced degeneration of CDs, increased caveolin-1 expression and p38 phosphorylation, up-regulated the mRNA level of IL-1 beta, TNF-alpha, MMP-3 and MMP-13. However, the IL-1 beta-induced activation of caveolin-p38 signaling and alteration in the expression of p38 downstream target genes were suppressed by ZGJXD-S and/or SB203580 in CDs.
ZGJXD can prevent CDs degeneration via inhibition of caveolin-p38 MAPK signal pathway, which might be one of the mechanisms that ZGJXD treats osteoarthritis.

• 出版日期2014-5
• 单位福建中医药大学