A Novel Hydrogen Peroxide Biosensor Based on Adsorption of Horseradish Peroxidase onto a Nanobiomaterial Composite Modified Glassy Carbon Electrode

作者：Moyo Mambo^*; Okonkwo Jonathan O; Agyei Nana M

来源：Electroanalysis, 2013, 25(8): 1946-1954.

DOI：10.1002/elan.201300165

• Hydrogen peroxide
• Adsorption
• Horseradish peroxidase
• Maize tassel
• Multiwalled carbon nanotube
• Glassy carbon electrode

In this work, a novel hydrogen peroxide biosensor derived from maize tassel (MT) and multiwalled carbon nanotube (MWCNT) composite was used to adsorb horseradish peroxidase (HRP) onto the surface of a glassy carbon electrode through electrostatic interactions. The morphology and structure of the products were characterized by SEM, FTIR and UV-visible spectroscopy. The electrochemical and electrocatalytic performance of the HRP/MT-MWCNT/GCE was studied using voltammetric and amperometric methods. The amperometric response of the biosensor varied linearly with concentration of H2O2 from 9 mu M to 1mM with detection limit of 4.0 mu M (S/N=3). Furthermore, the biosensor exhibited good reproducibility and stability.

• 出版日期2013-8