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摘要
PURPOSE: The aim of this study was to investigate the methylation status of three cell adhesion-related genes including CDH1, TSLC1 and TIMP3 in non-small cell lung cancer and explore its association with clinicopathologic features and various environmental risk factors. @@@ METHODS: We detected the aberrant methylation presence of these genes by methylation-specific polymerase chain reaction and analyzed the potential correlations with multivariate logistic regression model as well as stepwise logistic regression. @@@ RESULTS: For CDH1, promoter methylation was less frequent in adenosquamous carcinomas than adenocarcinomas (OR = 0.35, 95%CI = 0.13-0.96); pickled food increased the methylation frequency (OR = 2.23, 95%CI = 1.09-4.54) while light smoking and fruit intake decreased that (OR = 0.43, 95%CI = 0.19-0.97; OR = 0.37, 95%CI = 0.15-0.95). For TSLC1, males and toxin exposure increased methylation frequency (OR = 6.25, 95%CI = 1.05-37.13; OR = 2.42, 95%CI = 1.01-5.77) while light smoking and radiation exposure decreased that (OR = 0.14, 95%CI = 0.03-0.60; OR = 0.17, 95%CI = 0.04-0.87). For TIMP3, males showed lower methylation frequency than females (OR = 0.18, 95%CI = 0.04-0.88) while central lung cancer, heavy smoking and radiation exposure presented higher aberrant DNA methylation status (OR = 2.19, 95%CI = 1.07-4.52; OR = 6.99, 95%CI = 1.32-37.14; OR = 2.30, 95%CI = 1.04-5.08). @@@ CONCLUSIONS: Aberrant promoter methylation of cell adhesion-related tumor suppressor genes in lung cancer displayed varieties of gene-specific correlations with clinicopathologic features and various environmental risk factors.
- 出版日期2013
- 单位浙江大学
