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摘要
This paper examines the recovery of the enteric adenovirus human adenovirus 40 (HAdV 40) by cross-flow ultrafiltration and interprets recovery values in terms of physicochemical interactions of virions during sample concentration. Prior to ultrafiltration, membranes were either blocked by exposure to calf serum (CS) or coated with a polyelectrolyte multilayer (PEM). HAdV 40 is a hydrophobic virus with a point of zero charge between pH 4.0 and pH 4.3. In accordance with predictions from the extended Derjaguin-Landau-Verwey-Overbeek theory, the preelution recovery of HAdV (r(pre)) from deionized water was higher with PEM-coated membranes (r(pre)(PEM) = 74.8% +/- 9.7%) than with CS-blocked membranes (r(pre)(CS) = 54.1% +/- 6.2%). With either membrane type, the total virion recovery after elution (r(post)) was high for both deionized water (r(post)(PEM) = 99.5% +/- 6.6% and r(post)(CS) = 98.8% +/- 7.7%) and tap water (r(post)(PEM) = 89% +/- 15% and r(post)(CS) = 93.7% +/- 6.9%). The nearly 100% recoveries suggest that the polyanion (sodium polyphosphate) and surfactant (Tween 80) in the eluent disrupt electrostatic and hydrophobic interactions between the virion and the membrane. Addition of EDTA to the eluent greatly improved the elution efficacy (r(post)(CS) = 88.6% +/- 4.3% and r(post)(PEM) = 87.0% +/- 6.9%) with surface water, even when the organic carbon concentration in the water was high (9.4 +/- 0.1 mg/liter). EDTA likely disrupts cation bridging between virions and particles in the feed water matrix or the fouling layer on the membrane surface. For complex water matrices, the eluent composition is the most important factor for achieving high virion recovery. IMPORTANCE Herein we present the results of a comprehensive physicochemical characterization of HAdV 40, an important human pathogen. The data on HAdV 40 surface properties enabled rigorous modeling to gain an understanding of the energetics of virion-virion and virion-filter interactions. Cross-flow filtration for concentration and recovery of HAdV 40 was evaluated, with postelution recoveries from ultrapure water (99%), tap water (similar to 91%), and high-carbon-content surface water (similar to 84%) being demonstrated. These results are significant because of the very low adenovirus recoveries that have been reported, to date, for other methods. The recovery data were interpreted in terms of specific interactions, and the eluent composition was designed accordingly to maximize HAdV 40 recovery.
- 出版日期2016-8
