Development of a dual joystick-controlled laser trapping and cutting system for optical micromanipulation of chromosomes inside living cells

作者：Harsono Marcellinus S; Zhu Qingyuan; Shi Linda Z^*; Duquette Michelle; Berns Michael W

来源：Journal of Biophotonics, 2013, 6(2): 197-204.

DOI：10.1002/jbio.201200019

• laser trapping
• laser ablation
• optical micromanipulation
• robotic laser microscope system

A multi-joystick robotic laser microscope system used to control two optical traps (tweezers) and one laser scissors has been developed for subcellular organelle manipulation. The use of joysticks has provided a user-friendly method for both trapping and cutting of organelles such as chromosomes in live cells. This innovative design has enabled the clean severing of chromosome arms using the laser scissors as well as the ability to easily hold and pull the severed arm using the laser tweezers.

• 出版日期2013-2