Determination of temozolomide in serum and brain tumor with micellar electrokinetic capillary chromatography

作者：Andrasi Melinda; Toerzsoek Brigitta; Klekner Almos; Gaspar Attila^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2011, 879(23): 2229-2233.

DOI：10.1016/j.jchromb.2011.06.005

• Temozolomide
• MEKC
• Serum
• Brain
• Tumor

Micellar electrokinetic capillary chromatographic (MEKC) with photodiode-array detection was applied to determine temozolomide (TMZ) in human serum and brain tumor. The limit of guantitation (LOO) was 0.096 mu g/mL using 325 nm as detection wavelength. The method made possible that the TMZ could be detected in in vivo serum samples without sample pretreatment. In order to detect TMZ at lower concentration, an extraction with ethyl acetate was applied to preconcentrate the analyte. Small amount of brain tumor tissues (less than 1 g) were lyophilized and pretreated using extraction as a clean up and concentrating step. After removing the organic solvent a final sample volume of only 10 mu L was analyzed. The obtained peak concentrations (8.2-10.1 mu g/mL) and T(max) (44-65 min) of TMZ in serum were similar to the data reported by others, the in vivo TMZ concentrations found in brain tumor ranged between 0.061 and 0.117 mu g/g. 0 2011 Elsevier B.V.

• 出版日期2011-8-1