Aromatic interactions at the catalytic subsite of sucrose phosphorylase: Their roles in enzymatic glucosyl transfer probed with Phe(52) -> Ala and Phe(52) -> Asn mutants

作者：Wildberger Patricia; Luley Goedl Christiane; Nidetzky Bernd^*

来源：FEBS Letters, 2011, 585(3): 499-504.

DOI：10.1016/j.febslet.2010.12.041

• Sucrose phosphorylase
• GH-13
• Cation-pi interaction
• Oxocarbenium ion
• Aromatic stacking
• Glycoside hydrolase
• Glycosyltransferase

Mutants of Leuconostoc mesenteroides sucrose phosphorylase having active-site Phe(52) replaced by Ala (F52A) or Asn (F52N) were characterized by free energy profile analysis for catalytic glucosyl transfer from sucrose to phosphate. Despite large destabilization (>= 3.5 kcal/mol) of the transition states for enzyme glucosylation and deglucosylation in both mutants as compared to wild-type, the relative stability of the glucosyl enzyme intermediate was weakly affected by substitution of Phe(52). In reverse reaction where fructose becomes glucocylated, "error hydrolysis'' was the preponderant path of breakdown of the covalent intermediate of F52A and F52N. It is proposed, therefore, that Phe(52) facilitates reaction of the phosphorylase through (1) positioning of the transferred glucosyl moiety at the catalytic subsite and (2) strong cation-p stabilization of the oxocarbenium ion-like transition states flanking the covalent enzyme intermediate.

• 出版日期2011-2-4