Differential gene expression of proinflammatory chemokines and cytokines in lungs of ascites-resistant and -susceptible broiler chickens following intravenous cellulose microparticle injection

作者：Hamal Krishna R; Wideman Robert F; Anthony Nicholas B; Erf Gisela F^*

来源：Veterinary Immunology and Immunopathology, 2010, 133(2-4): 250-255.

DOI：10.1016/j.vetimm.2009.07.011

• Pulmonary hypertension syndrome
• Broilers
• Inflammation
• Cytokine production
• Chemokine production
• Perivascular mononuclear cell aggregates
• Intravenous microparticle injection

Intravenous injection of microparticles (MPs) is a tool to reveal susceptibility to pulmonary hypertension (PH) syndrome (PHS, ascites) in broilers. After injection MPs get lodged in pulmonary arterioles and cause localized inflammation. To examine the expression of chemokines/cytokines during the MP-induced pulmonary inflammatory response, lungs were collected from 4-week-old broilers (6/line/time point) from the PHS-resistant (RES) and -susceptible (SUS) broilers before (0 h) and after (2, 6, 12, 24, and 48 h) MP injection and analyzed using quantitative RT-PCR. In both lines, expression of interleukin-1 beta (IL-1 beta), IL-6, IL-8, and K60 increased from 0 to 6 h, reached peak levels at 6 and 12 h, and decreased thereafter, whereas IL-4 and interferon gamma (IFN-gamma) expression remained elevated past 12 h. Lungs from the RES line broilers had higher expression (P < 0.05) of IL-1 beta and IL-6 at 2, 6, and 12 h; higher IL-8 at 6 and 12 h; higher K60 at 6,12, and 24 h; higher IL-4 at 12,24, and 48 h and higher IFN-gamma expression at 6 and 48 h post-MP injection than SUS line broilers. Higher expression of chemokines/cytokines in RES compared to SUS line lungs may explain the ability of RES line broilers to effectively counteract the MP-induced PH and resolve the vascular occlusion.

• 出版日期2010-2-15