N-acetyltransferase 1 polymorphism increases cotinine levels in Caucasian children exposed to secondhand smoke: the CCAAPS birth cohort

作者：LeMasters G K; Hershey G K Khurana; Sivaprasad U; Martin L J; Pilipenko V; Ericksen M B; Burkle J W; Lindsey M A; Bernstein D I; Lockey J E; Gareri J; Lubetsky A; Koren G; Myers J M Biagini^*

来源：The Pharmacogenomics Journal, 2015, 15(2): 189-195.

DOI：10.1038/tpj.2014.44

Cotinine is a proxy for secondhand smoke (SHS) exposure. Genetic variation along nicotine and cotinine metabolic pathways may alter the internal cotinine dose, leading to misinterpretations of exposure-health outcome associations. Caucasian children with available SHS exposure and hair cotinine data were genotyped for metabolism-related genes. SHS-exposed children had 2.4-fold higher hair cotinine (0.14 +/- 0.22 ng mg(-1)) than unexposed children (0.06 +/- 0.05 ng mg(-1), P < 0.001). SHS-exposed children carrying the NAT1 minor allele had twofold higher hair cotinine (0.18 ng mg(-1) for heterozygotes and 0.17 ng mg(-1) for homozygotes) compared with major allele homozygotes (0.09 ng mg(-1), P = 0.0009), even after adjustment for SHS dose. These findings support that NAT1 has a role in the metabolic pathway of nicotine/cotinine and/or their metabolites. The increased cotinine levels observed for those carrying the minor allele may lead to SHS exposure misclassification in studies utilizing cotinine as a biomarker. Additional studies are required to identify functional single-nucleotide polymorphism(s) (SNP(s)) in NAT1 and elucidate the biological consequences of the mutation(s).

• 出版日期2015-4