DOWNHILL SEARCH-BASED NRL GLOVE PROTEIN ESTIMATION

作者：Ting H Y^*; Yong K P; Mok K L; Sim K S

来源：Rubber Chemistry and Technology, 2013, 86(4): 653-663.

DOI：10.5254/rct.13.87957

Latex allergy is still a crucial concern for latex glove users and manufacturers. The major reason is that the possibility of allergy stems from the soluble proteins that reside within latex gloves that may be transferred into the body of the user. Conventionally, the protein level quantification by the colorimetric measurement test is usually complex and requires along processing time, sophisticated equipment, and highly trained specialists to perform the test. Because of the costly, lengthy, and labor-intensive nature of the tests, these methods are not beneficial for the efficiency of product development. Hence, a new computerized measurement method is proposed to measure protein levels. The prepared latex glove samples were chemically treated by Bradford reagent. Then, the samples were scanned and analyzed by novel algorithm, namely, the downhill searching algorithm (DSA), to determine the dominant color intensity. A total of 108 data were obtained after DSA analysis, and the developed data were validated with protein concentration data provided by the Malaysian Rubber Board. A good correlation is observed between both data.

• 出版日期2013-12