A DNA-topoisomerase-II-binding protein with eight repeating regions similar to DNA-repair enzymes and to a cell-cycle regulator

作者：Yamane K; Kawabata M; Tsuruo T^*

来源：European Journal of Biochemistry, 1997, 250(3): 794-799.

DOI：10.1111/j.1432-1033.1997.00794.x

• topoisomerase
• two-hybrid system
• cleavable complex
• Cut5
• DNA repair

A two-hybrid system was used to isolate factors that interact with the C-terminal region of DNA topoisomerase II beta A positive clone isolated from a HeLa cDNA library encoded 1522 amino acid residues (molecular mass 170670). The protein, designated topoisomerase-II beta-binding protein 1 (TopBP1). interacted with the C-terminal region of topoisomerase II beta synthesized in vitro. A database search indicated that TopBP1 possessed eight regions similar to regions of Rad4, Cut5, Ect2, Rev 1 and X-ray repair cross-complementing 1 (XRCC1) proteins and a region similar to auto-modification sites of poly(ADP-ribose) polymerase, suggesting that TopBP1 supported catalytic reactions of topoisomerase II through transient breakages of DNA strands.

• 出版日期1997-12-15