Airlift bioreactor system and nitrogen sources for biomass and antioxidant compound production from in vitro culture of Vitis flexuosa plantlets

作者：Park Jeong Ae; Park Byung Jun; Kim A Hyun; Park So Young^*; Paek Kee Yoeup

来源：Horticulture Environment and Biotechnology, 2015, 56(3): 358-365.

DOI：10.1007/s13580-015-0006-4

• DPPH
• flavonoids
• NH4+: NO3- ratio
• phenolics
• temporary immersion bioreactor

This study was performed to establish an improved and effective shoot culture system using a bioreactor for the commercial biomass production from whole plants of Vitis flexuosa. To determine the effect of different medium supplement systems on biomass and bioactive compound accumulation, in vitro shoots were cultured in several different medium supplement systems [solid culture (conventional method), liquid culture (control), continuous immersion bioreactor (CIB), temporary immersion bioreactor (TIB), CIB with net (CIB-N), and TIB with net (TIB-N)]. To investigate the optimal NH4 (+) and NO3 (-) ratio in medium, in vitro shoots were cultured with NH4 (+) and NO3 (-) ratios of 0:30, 10:20, 15:15, 20:10, and 30:0 mM in 1/2 MS medium. After 4 weeks of culture, the CIB system produced the highest biomass productivity (43.80 g center dot L-1 FW and 10.11% DW) but showed poor plant development. In comparison with CIB, the plantlet growth was improved in the TIB system in terms of plant height, leaf number, and root length. A greater quantity of total phenolics was also recorded in the TIB system as compared to the CIB system, whereas flavonoid content was similar in both systems. A 10:20 mM NH4 (+):NO3 (-) ratio in the TIB system medium showed an increase of biomass (50.99 g center dot L-1 medium FW, 4.37 g center dot L-1 medium DW) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (89.09%). Bioactive compound accumulation increased with increasing NO3 (-) concentration, with the highest levels being obtained from the 10:20 mM NH4 (+):NO3 (-) ratio after 4 weeks of culture. After 7 days of elicitation with 100-mu M methyl jasmonate, the increased percentage of bioactive compounds and DPPH radical scavenging activity was obtained under the 15:15 mM NH4 (+):NO3 (-) ratio treatment. The present study indicated that the proper selection of a bioreactor system and manipulation of the NH4 (+) and NO3 (-) ratio, along with the elicitation of the shoot culture, led to the rapid production of sufficient biomass and bioactive compounds in V. flexuosa.

• 出版日期2015-6