Fabrication of aptamer decorated dextran coated nano-graphene oxide for targeted drug delivery

作者：Alibolandi Mona; Mohammadi Marzieh; Taghdisi Seyed Mohammad; Ramezani Mohammad; Abnous Khalil

来源：Carbohydrate Polymers, 2017, 155: 218-229.

DOI：10.1016/j.carbpol.2016.08.046

• Graphene oxide
• Dextran
• AS1411
• Curcumin

In the current study, dextran (DEX) was covalently conjugated to the surface of nano-GO sheets, making stable biocompatible dextran coated GO (GO-DEX). The prepared GO-DEX was nontoxic to 4T1 mammary carcinoma cell line at concentrations up to 300 mu g/mL.
AS1411 aptamer, a ssDNA aptamer which can improve the intracellular uptake by nucleolin recognition, also has been introduced to hydroxyl groups of DEX in GO-DEX to produce GO-DEX-Apt. Moreover, curcumin (CUR), a natural polyphenol, found in the rhizomes of Curcuma Tonga (turmeric) which shows antineoplastic effects, was loaded onto the GO-DEX and GO-DEX-Apt via pi-pi stacking interactions with a high loading capacity (similar to 29 wt%). The GO-DEX-Apt-CUR could efficiently enter into 4T1 and MCF-7 nucleolin over-expressed cancer cells confirmed by fluorescence microscope and flowcytometry, and it also showed significantly higher cytotoxicity. These types of targeted nanoscale drug delivery vehicles on the basis of DEX coated GO may find potential application in cancer chemotherapy.

• 出版日期2017-1-2