科研之友
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摘要
High-throughput molecular profiling techniques are helpful in the diagnosis of multifactorial disease. In this study, a cDNA-phage-displayed protein microarray using phage particles spotted directly onto it as sensors was used to detect related antigens in breast tumor sera. cDNA sequences from 17 positive clones were determined, which included some sequences encoding known breast cancer-related antigens and proteins related to other diseases, as well as proteins with unknown functions. Our results not only provide some useful information for breast cancer research, but also suggest that the strategy used here would be efficient to search for disease-related proteins and other functional target proteins.
- 出版日期2010-7
- 单位北京大学; 北京市肿瘤防治研究所
