Antimicrobial and Antioxidant Activities of the Root Bark Essential Oil of Periploca sepium and Its Main Component 2-Hydroxy-4-methoxybenzaldehyde

作者：Wang Jihua; Liu Hao; Zhao Jianglin; Gao Haifeng; Zhou Ligang^*; Liu Zhilong; Chen Yuanquan; Sui Peng

来源：Molecules, 2010, 15(8): 5807-5817.

DOI：10.3390/molecules15085807

• Asclepiadaceae
• Apocynaceae
• Periploca sepium
• essential oil
• 2-hydroxy-4-methoxybenzaldehyde
• antimicrobial activity
• antioxidant activity

The root bark essential oil of Periploca sepium Bunge (Asclepiadaceae/Apocynaceae) obtained by hydrodistillation was investigated by GC and GC-MS. 2-Hydroxy-4-methoxybenzaldehyde was found to be the main component (78.8% of the total) among 17 identified compounds. 2-Hydroxy-4-methoxybenzaldehyde was separated and purified by preparative thin layer chromatography, and was further identified by means of physicochemical and spectrometric analysis. Both the essential oil and 2-hydroxy-4-methoxybenzaldehyde exhibited antimicrobial activities on all test bacteria and fungi, with MBC/MFC values ranging from 125 mu g/mL to 300 mu g/mL, MIC values from 80 mu g/mL to 300 mu g/mL, and IC(50) values from 63.29 mu g/mL to 167.30 mu g/mL. They also showed moderate antioxidant activity in the DPPH free radical scavenging, beta-carotene bleaching and ferrozine-ferrous ions assays. The results indicated that the major portion of these antimicrobial and antioxidant activities was due to the presence of 2-hydroxy-4-methoxybenzaldehyde in the oil, which could potentially be developed as an antimicrobial and antioxidant agent in the future.

• 出版日期2010-8
• 单位中国农业大学