Association of p.His38Leu, a Rare CYP21A2 Mutation, with the Classical Simple Virilizing Phenotype of 21-Hydroxylase Deficiency in a 6-Year-Old Boy

作者：Oriola Josep^*; Bosch Maria Zelmira; Valls Carme; Ibanez Lourdes

来源：Hormone Research in Paediatrics, 2011, 76(3): 214-217.

DOI：10.1159/000327366

• 21-hydroxylase deficiency
• Congenital adrenal hyperplasia
• p.His38Leu mutation
• Simple virilizing phenotype
• CYP21A2 mutation

Background/Aims: Congenital adrenal hyperplasia due to 21-hydroxylase deficiency is one of the most common autosomal recessive disorders. Unequal crossing over or gene conversion between the active CYP21A2 gene and the inactive CYP21A1P pseudogene accounts for 95% of mutations, but many non-pseudogene-derived mutations have been described. It is important for these new mutations to be assigned to a specific phenotype. Case Report and Results: We report a young boy diagnosed with the classical simple virilizing phenotype in whom the CYP21A2 genetic analysis disclosed that he was a compound heterozygous for p.His38Leu and the c.290-13A/C>G mutations. The p.His38Leu mutation has been recently described, but has not been associated with a specific phenotype thus far. Residue 38 is the only charged amino acid of a hydrophobic patch that interacts closely with the membrane. This mutation leads to a non-charged amino acid, suggesting a much more hydrophobic continuous domain. This patch is highly conserved through different mammals indicating the importance of this domain in the protein-membrane interaction. Conclusion: Our results support p.His38Leu as a severe mutation causing a classical simple virilizing phenotype.

• 出版日期2011