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摘要
An electrochemical immunosensor for ultrasensitive detection of acrylamide (AA) in water and food samples was developed. SnO2-SiC hollow sphere nanochains with high surface area and gold nano-particles with good electroconductivity were fabricated onto the surface of a glassy carbon electrode pre-coated with chitosan. The coating antigen (AA-4-mercaptophenylacetic acid-ovalbumin conjugate, AA-4-MPA-OVA) was immobilized on the electrode. Polyclonal antibody specific for AA-4-MPA was conjugated to gold nanorod (AuNR) as primary antibody (AuNR-Ab(1)). Horseradish peroxidase labelled anti-rabbit antibody produced in goat was conjugated to AuNR as secondary antibody (HRP-AuNR-Ab(2)). For detection, the analyte (AA-4-MPA) in sample competed with coating antigen for binding with AuNR-Ab1. After washing, HRP-AuNR-Ab(2) was added to capture the AuNR-Ab(1), and the electrical signal was obtained by addition of hydroquinone and H2O2. After investigation of the binding ability on nanomaterials and optimization of competitive immunoassay conditions, the proposed immunosensor exhibited a sensitive response to AA with a detection limit of 45.9 +/- 2.7 ng kg(-1), and working range of 187 +/- 12.3 ng kg(-1) to 104 +/- 8.2 mu g kg(-1) for drinking water samples. Recoveries of AA from spiked samples were ranged from 86.0% to 115.0%. The specificity, repeatability and stability of the immunosensor were also proved to be acceptable, indicating its potential application in AA monitoring.
- 出版日期2019-2-21
- 单位华南农业大学; 华南热带农业大学
