A dual functional peptide carrying in vitro selected catalytic and binding activities

作者：Zhu, Liping; Wang, Wei; Zhao, Haixu; Xu, Muye; Tada, Seiichi; Uzawa, Takanori; Liu, Mingzhe; Ito, Yoshihiro^*

来源：Organic and Biomolecular Chemistry, 2015, 13(38): 9808-9812.

DOI：10.1039/c5ob01271f

• HEME-CONTAINING OXYGENASES
• PEROXIDASE-ACTIVITY
• DNA APTAMER
• COMPLEX
• RNA
• PORPHYRINS
• EVOLUTION
• PROBE

When minimal functional sequences are used, it is possible to integrate multiple functions on a single peptide chain, like a "single stroke drawing". Here a dual functional peptide was designed by combining in vitro selected catalytic and binding activities. For catalytic activity, we performed in vitro selection for a peptide aptamer binding to hemin by using ribosome display and isolated a peptide that had peroxidase activity in the presence of hemin. By combining the selected catalytic peptide with a peptide antigen, which can be recognized by an antibody, an enzyme-antibody conjugate-like peptide was obtained. This study demonstrates a successful strategy to create dual functionalized peptide chains for use in immunoassays.

• 出版日期2015
• 单位RIKEN; 沈阳药科大学