Analysis of endogenous aldehydes in human urine by static headspace gas chromatography-mass spectrometry

作者：Serrano Maria; Gallego Mercedes^*; Silva Manuel

来源：Journal of Chromatography A, 2016, 1437: 241-246.

DOI：10.1016/j.chroma.2016.01.056

• Endogenous aldehydes
• Static headspace technique
• Gas chromatography-mass spectrometry
• Human urine
• Oxidative stress

Endogenous aldehydes (EAs) generated during oxidative stress and cell processes are associated with many pathogenic and toxicogenic processes. The aim of this research was to develop a solvent-free and automated analytical method for the determination of EAs in human urine using a static headspace generator sampler coupled with gas chromatography mass spectrometry (HS-GC-MS). Twelve significant EAs used as markers of different biochemical and physiological processes, namely short- and medium-chain alkanals, alpha,beta-unsaturated aldehydes and dicarbonyl aldehydes have been selected as target analytes. Human urine samples (no dilution is required) were derivatized with O-2,3,4,5,6-pentafluorobenzylhydroxylamine in alkaline medium (hydrogen carbonate carbonate buffer, pH 10.3). The HS-GC-MS method developed renders an efficient tool for the sensitive and precise determination of EAs in human urine with limits of detection from 1 to 15 ng/L and relative standard deviations, (RSDs) from 6.0 to 7.9%. Average recoveries by enriching urine samples ranged between 92 and 95%. Aldehydes were readily determined at 0.005-50 mu g/L levels in human urine from healthy subjects, smokers and diabetic adults.

• 出版日期2016-3-11