Determination of 11 Fat-soluble Vitamins (A, D, E, K) and Their Derivatives in Vitamin Tablets by Ultra Performance Convergence Chromatography

作者：Zhou Wei^*; Wang Bo; Liu Qian-Qian; Yang Sheng-Xin; Wang Li-Ting

来源：Chinese Journal of Analytical Chemistry, 2015, 43(1): 115-120.

• Ultra performance convergence chromatography
• Fat-soluble Vitamin
• Vitamin tablets

A new method was developed for the determination of 11 fat-soluble vitamins ( A, D, E and K) and its derivatives in vitamin tablets by ultra performance convergence chromatography ( UPC2). The mobile phase was the mixture of supercritical CO2 and acetonitrile at a flow rate of 1 mUmin. The separation was carried out on the Waters Acquity UPC2 HSS C-18 SB 100 mm x 3. 0 mm i. d., 1. 8 mu m column. The UV detector was set at a wavelength of 284 nm. The limits of detection ( LOD) were 1. 5-2. 0 mg/L, and the calibration linear for VK1 VK2, VK3 and VB3 was 3-300 mg/L, linear for VA, VA palmitate, VA formic acid, VE, VE acetate, VD2 and VD, was 5-300 mg/L, respectively. Its spiked recoveries were 97. 31% - 98.76%, and the relative standard deviations ( RSDs) were 0.41% 0. 96%. The method is applicable for the determination of fat-soluble vitamins ( A, D, E and K) and Their derivatives in vitamin tablets.

• 出版日期2015-1
• 单位西北师范大学; 甘肃农业大学