A facile approach toward protein-resistant biointerfaces based on photodefinable poly-p-xylylene coating

作者：Su Chiao Tzu; Yuan Ruei Hung; Chen Yung Chih; Lin Ting Ju; Chien Hsiu Wen; Hsieh Chih Chen; Tsai Wei Bor; Chang Chih Hao^*; Chen Hsien Yeh

来源：Colloids and Surfaces B: Biointerfaces , 2014, 116: 727-733.

DOI：10.1016/j.colsurfb.2013.11.044

• Surface modifications
• CVD polymerization
• Protein adsorptions
• Biointerface
• Antifouling
• Photopatterning

A facile and versatile tool is reported that uses a photodefinable polymer, poly(4-benzoyl-p-xylylene-co-p-xylylene) to immobilize antifouling materials, such as poly(ethylene glycol), poly(ethylene glycol) methyl ether methacrylate, dextran, and ethanolamine. This immobilization process requires the polymer's photoactivated carbonyl groups, which can facilitate light-induced molecular crosslinking and can rapidly react via insertion into C-H or N-H bonds upon photo-illumination at 365 nm. Importantly, the process does not require additional functional groups on the antifouling materials. The immobilized fouling materials were characterized using X-ray photoelectron spectroscopy (XPS) and infrared reflection absorption spectroscopy (IRRAS), and the resulting antifouling properties were examined through protein adsorption studies on fibrinogen and bovine serum albumin at surfaces that were spatially modified using a photomask during the photochemical process. In addition, the adsorbed fibrinogen was quantitatively analyzed using a quartz crystal microbalance (QCM), and the adsorption values were reduced to 32.8 +/- 14.9 ng cm(-2), 5.5 +/- 13.9 ng cm(-2), 21.4 +/- 4.5 ng cm(-2), and 16.9 +/- 3.4 ng cm-(2) for poly(ethylene glycol) (PEG), poly(ethylene glycol) methyl ether methacrylate (PEGMA), dextran, and ethanolamine, respectively. Finally, this antifouling modification technology was demonstrated on an unconventional substrate for a stent that was modified by PEGMA at selected areas using a microscopic patterning technique during photoimmobilization. Low levels of fibrinogen and BSA adsorption were also observed at the areas where PEGMA was attached.

• 出版日期2014-4-1