A thorough survey for Cr-resistant and/or -reducing bacteria identified comprehensive and pivotal taxa

作者：Huang, Yili^*; Feng, Hao; Lu, Hang; Zeng, Yanhua

来源：International Biodeterioration & Biodegradation, 2017, 117: 22-30.

DOI：10.1016/j.ibiod.2016.11.010

• Cr-resistant
• Cr-reducing
• Genome mining
• Diversity
• Survey

To better understand the bacterial potential in chromium (Cr) bioremediation, a thoroughly survey was performed to investigate the diversity and importance of Cr-resistant and/or -reducing bacterial taxa by genome mining, literature survey and laboratory isolation. Genome mining among 7887 species revealed that 1877 species distributed in 20 phyla contained Cr-resistant gene chrA. Pseudomonas, Bacillus and Vibrio were the three most abundant genera. 81 species affiliated to 4 phyla were found containing Cr(VI) reductase gene. Bifidobacterium, Bordetella and Bacillus were the three most abundant genera. Importantly, genome mining identified 30 genera contained both chrA and Cr(VI) reductase genes, while literature survey showed that 43 genera capable of Cr(VI) resistant and reducing. The two sets taxa have only four genera in overlap. We conducted an isolation work in lab in which nine Cr(VI)-resistant bacteria and one Cr(VI)-reducing Arthrobacter were isolated from xixi wetland sediment. Eight isolates fell in taxa identified by genome mining or literature survey, while Lactococcus represented a novel Cr(VI)-resistant genus, indicating that there are more Cr(VI)-resistant and/or-reducing taxa in the nature to be recognized. We present here the so-far most comprehensive Cr-resistant and/or -reducing bacterial taxa, which provide important information for Cr bioremediation.

• 出版日期2017-2
• 单位浙江大学