Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells

作者：Mittelbrunn Maria; Gutierrez Vazquez Cristina; Villarroya Beltri Carolina; Gonzalez Susana; Sanchez Cabo Fatima; Angel Gonzalez Manuel; Bernad Antonio; Sanchez Madrid Francisco^*

来源：Nature Communications, 2011, 2(1): 282.

DOI：10.1038/ncomms1285

The immune synapse is an exquisitely evolved means of communication between T cells and antigen-presenting cells (APCs) during antigen recognition. Recent evidence points to the transfer of RNA via exosomes as a novel mode of intercellular communication. Here we show that exosomes of T, B and dendritic immune cells contain microRNA (miRNA) repertoires that differ from those of their parent cells. We investigate whether miRNAs are exchanged during cognate immune interactions, and demonstrate the existence of antigen-driven unidirectional transfer of miRNAs from the T cell to the APC, mediated by the delivery of CD63(+) exosomes on immune synapse formation. Inhibition of exosome production by targeting neutral sphingomyelinase-2 impairs transfer of miRNAs to APCs. Moreover, miRNAs transferred during immune synapsis are able to modulate gene expression in recipient cells. Thus, our results support a mechanism of cellular communication involving antigen-dependent, unidirectional intercellular transfer of miRNAs by exosomes during immune synapsis.

• 出版日期2011-4