Anti-inflammatory effects of Perilla frutescens in activated human neutrophils through two independent pathways: Src family kinases and Calcium

作者：Chen Chun Yu; Leu Yann Lii; Fang Yu; Lin Chwan Fwu; Kuo Liang Mou; Sung Wei Che; Tsai Yung Fong; Chung Pei Jen; Lee Ming Chung; Kuo Yu Ting; Yang Hsuan Wu; Hwang Tsong Long^*

来源：Scientific Reports, 2015, 5(1): 18204.

DOI：10.1038/srep18204

The leaves of Perilla frutescens (L.) Britt. have been traditionally used as an herbal medicine in East Asian countries to treat a variety diseases. In this present study, we investigated the inhibitory effects of P. frutescens extract (PFE) on N-formyl-Met-Leu-Phe (fMLF)-stimulated human neutrophils and the underlying mechanisms. PFE (1, 3, and 10 mu g/ml) inhibited superoxide anion production, elastase release, reactive oxygen species formation, CD11b expression, and cell migration in fMLF-activated human neutrophils in dose-dependent manners. PFE inhibited fMLF-induced phosphorylation of the Src family kinases (SFKs), Src (Tyr416) and Lyn (Tyr396), and reduced their enzymatic activities. Both PFE and PP2 (a selective inhibitor of SFKs) reduced the phosphorylation of Burton's tyrosine kinases (Tyr223) and Vav (Tyr174) in fMLF-activated human neutrophils. Additionally, PFE decreased intracellular Ca2+ levels ([Ca2+](i)), whereas PP2 prolonged the time required for [Ca2+] i to return to its basal level. Our findings indicated that PFE effectively regulated the inflammatory activities of fML-Factivated human neutrophils. The anti-inflammatory effects of PFE on activated human neutrophils were mediated through two independent signaling pathways involving SFKs (Src and Lyn) and mobilization of intracellular Ca2+.

• 出版日期2015-12-14
• 单位长春大学