A BODIPY-based fluorescent chemosensor for Cu2+ and biological thiols, and its application as a Cu2+ probe in live cell imaging

作者：Zhao, Chun-Chao; Chen, Yong; Zhang, Hong-Yan; Zhou, Bing-Jiang; Lv, Xiao-Jun; Fu, Wen-Fu^*

来源：Journal of Photochemistry and Photobiology A: Chemistry , 2014, 282: 41-46.

DOI：10.1016/j.jphotochem.2014.03.009

• BODIPY
• Fluorescence quenching
• Cu2+ Sensor
• Biothiols sensor
• Cell images

A new fluorescent boron dipyrromethene (Bodipy) derivative 1 containing a di(2-picolyl)amine group as a binding site for Cu2+ was synthesized and characterized. Compound 1 behaves as an "on off" fluorescent sensor for highly selective and sensitive detection of Cu2+ ions. The selective interaction between compound I and Cu2+ leads to formation of a 1 center dot Cu2+ complex, associated with quenching of the fluorescence of 1. An investigation into the detection of biothiols shows that the 1 center dot Cu2+ complex has better recognition for glutathione than cysteine and homocysteine in CH3OH-HEPES (1:1, v/v) buffer solution at pH 7.4 (HEPES = 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid). In addition, fluorescence images obtained via confocal microscopy reveal that compound 1 exhibits excellent selectivity and high sensitivity for Cu2+ under physiological conditions and in living cells.

• 出版日期2014-5-15
• 单位中国科学院大学; 中国科学院理化技术研究所; 云南师范大学