Detection of nosocomial Clostridium difficile infections with toxigenic strains despite negative toxin A and B testing on stool samples

作者：Stahlmann J; Schoenberg M; Herrmann M; von Mueller L

来源：Clinical Microbiology and Infection, 2014, 20(9): O590-O592.

DOI：10.1111/1469-0691.12558

• Clostridium difficile
• GDH
• slpA sequence typing
• toxigenic culture
• Toxin

A two-step diagnostic algorithm is recommended to detect Clostridium difficile infections; however, samples are regularly found that are glutamate dehydrogenase (GDH) positive but stool toxin negative. In the present single-centre prospective study we focused on these difficult-to-interpret%26apos; samples and characterized them by anaerobic culture, toxigenic culture, slpA sequence typing and multiplex PCR (GenoType CDiff). The majority of stool toxin A and B-negative samples have been caused by toxigenic strains including ribotype 027. The multiplex PCR was faster and more sensitive compared with culture and allowed preliminary identification of hypervirulent strains in stool samples on the same day.

• 出版日期2014-9
• 单位河北医科大学