科研之友
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摘要
Gram-negative rods, particularly members of the Enterobacteriaceae and non-Enterobacteriaceae were the most commonly isolated organisms from poultry intestinal tract in 99 samples. Their prevalence, accurate identification and many biochemical tests had been determined using Analytical profile index (API 20E); (BioMe%26apos;rieux, Marcy l%26apos;Etoile, France), which were performed with 60 isolates of gram-negative rods, including 7 genera of Enterobacteriaceae (10 species). With supplemental testing, API, correctly identified to the genera and species of Enterobacteriaceae group as follows: 35 isolates of Escherishia coli 1 (58.33%), 6 isolates of Salmonella arizonae (10%), 6 isolates of Enterobacter cloacae (10%), 4 isolates of Kluyvera sp. (6.66%), 2 isolates of Enterobacter aerogenes (3.33%), 2 isolates of Klebsiella pneumoniae subsp. pneumoniae (3.33%), 1 isolate of Cedecea lapagei (1.66%), 1 isolate of Lelercia adecarboxylate (1.66%) and 1 isolate of Klebsiella oxycota (1.66%) and only 2 isolates of non-Enterobacteriaceae belonging to Aeromonas hydrophilla 1 (3.33%). In this study, zone of inhibitions (in mm) of the antibiotics on the test microorganisms were determined and interpreted using standard interpretative chart. Enterobacter aerogenes exhibited high resistance to the tested antibiotics with resistance percentage of 94.11% followed by Klebsiella pneumoniae subsp. pneumoniae and Leclercia adecarboxylata (88.23%), Enterobacter cloacae and Kluyvera sp. (57.82%), Aeromonas hydrophilla 1 and Cedecea lapagei (47.07%). Escherishia coli 1, Salmonella arizonae and Klebsiella oxycota were the lowest resistant to antibiotics and the resistance percentages were 41.17, 35.29 and 29.41%, respectively. The aim of the study is to determine the prevalence Enterobacteriaceae and non Enterobacteriaceae species of poultry intestinal tract as well as determine its antimicrobial resistance patterns.
- 出版日期2013
