Direct electrochemistry and electrocatalysis of horseradish peroxidase immobilized in hyaluronic acid and single walled carbon nanotubes composite film

作者：Wang Qi^*; Zheng Jianbin

来源：Chemical Papers, 2010, 64(5): 566-572.

DOI：10.2478/s11696-010-0053-3

• horseradish peroxidase
• single walled carbon nanotubes
• hyaluronic acid
• direct electrochemistry
• electrocatalysis

Direct electrochemistry and electrocatalysis of horseradish peroxidase (HRP) immobilized on a hyaluronic acid (HA)-single walled carbon nanotubes (SCNs) composite film coated glassy carbon electrode (GCE) was studied for the first time. HRP entrapped in the SCNs-HA composite film exhibited a pair of well-defined, quasi-reversible cyclic voltammetric peaks in a 0.1 M phosphate buffer solution (pH 7.0). Formal potential vs. standard calomel electrode (EA degrees') was -0.232 V, and EA degrees aEuro(2) was linearly dependent on the solution pH indicating that the electron transfer was proton-coupled. The current is linearly dependent on the scan rate, indicating that the direct electrochemistry of HRP in that case is a surface-controlled electrode process. UV-VIS spectrum suggested HRP retained its original conformation in the SCNs-HA film. Immobilized HRP showed excellent electrocatalysis in the reduction of hydrogen peroxide (H(2)O(2)).

• 出版日期2010-10
• 单位西安电子科技大学; 西北大学