Diversity of a mesophilic lignocellulolytic microbial consortium which is useful for enhancement of biogas production

作者：Yan, Lei^*; Gao, Yamei; Wang, Yanjie; Liu, Quan; Sun, Zhiyuan; Fu, Borui; Wen, Xue; Cui, Zongjun; Wang, Weidong

来源：Bioresource Technology, 2012, 111: 49-54.

DOI：10.1016/j.biortech.2012.01.173

• Mesophilic microbial community
• Lignocellulose degradation
• Rice straw
• Biogas
• 16S rRNA clone library

A mesophilic lignocellulolytic microbial consortium BYND-5, established by successive subcultivation, was applied to enhance the biogas production. The degradation efficiency of BYND-5 for rice straw was more than 49.0 +/- 1.8% after 7 days of cultivation at 30 degrees C. Various organic compounds, including acetic acid, propionic acid, butyric acid and glycerin were detected during biodegradation. The diversity analysis of BYND-5 was conducted by ARDRA (Amplified ribosomal DNA restriction analysis) of the 16S rDNA clone library. Results indicated that bacterial groups represented in the clone library were the Firmicutes (5.96%), the Bacteroidetes (40.0%), Deferribacteres (8.94%), Proteobacteria (16.17%), Lentisphaerae (2.13%), Fibrobacteraceae (1.7%), and uncultured bacterium (25.1%). Additionally, the enhancement of biogas yield and methane content was directly related to the pretreatment with BYND-5. The microbial community identified herein is potential candidate consortium for the degradation of waste lignocellulose and enhancement of biogas production under the mesophilic temperature conditions.

• 出版日期2012-5
• 单位黑龙江八一农垦大学; 中国农业大学