Methods for the evaluation of antibiotic resistance in Lactobacillus isolated from fermented sausages

作者：Wolupeck Hanna Lethycia; Morete Crisley Aparecida; DallaSanta Osmar Roberto; Luciano Fernando Bittencourt; Franca Madeira Humberto Maciel; Freitas de Macedo Renata Ernlund

来源：Ciencia Rural, 2017, 47(8): e20160966.

DOI：10.1590/0103-8478cr20160966

• meat product
• multiplex PCR
• probiotic
• susceptibility

The present study aimed to assess the antibiotic resistance in 54 indigenous Lactobacillus plantarum isolated from artisanal fermented sausages. The confirmation of the strain species was performed by multiplex-PCR assay. Antibiotic resistance was assessed by disk diffusion (DD) and Minimum Inhibitory Concentration (MIC) methods. Of 54 L. plantarum, 44 strains were genotypically confirmed as L. plantarum and 3 as Lactobacillus pentosus. The highest resistance rates were to ampicillin and streptomycin. The highest susceptibility rates were shown to tetracycline, chloramphenicol and penicillin G. None of the strains showed multidrug resistance. Resistance rates by DD and MIC were not different ( P > 0.05) for ampicillin, chloramphenicol, erythromycin and penicillin G. Future research should assess the genetic mechanisms underlying the phenotypic resistance in Lactobacillus strains to screen the potential probiotic strains for the development of functional meat products.

• 出版日期2017