Antihypertensive and antioxidant effects of supplementation with red wine pomace in spontaneously hypertensive rats

作者：Del Pino-Garcia, Raquel; Rivero-Perez, Maria D.; Gonzalez-SanJose, Maria L.; Croft, Kevin D.; Muniz, Pilar^*

来源：Food & Function, 2017, 8(7): 2444-2454.

DOI：10.1039/c7fo00390k

• INDUCED ENDOTHELIAL DYSFUNCTION
• BLOOD-PRESSURE
• MOLECULAR-MECHANISMS
• MASS-SPECTROMETRY
• HEME OXYGENASE-1
• NITRIC-OXIDE
• IN-VIVO
• POLYPHENOLS
• PLASMA
• DISEASE

Hypertension is associated with enhanced vascular oxidative stress and impaired endothelial function, which is related to an imbalance between reactive oxygen species and nitric oxide bioavailability. Shortterm supplementation with a polyphenol-rich powdered red wine pomace seasoning (RWPS) was investigated for its effects on blood pressure and biomarkers of endothelial dysfunction and oxidative status in a model of essential hypertension. Male spontaneously hypertensive rats (SHRs) and normotensive Wistar-Kyoto rats (12-week-old, 5 rats per group) were administered RWPS (300 mg kg(-1) day(-1); equivalent to 7.32 mg gallic acid per kg per day) or vehicle by gavage. In SHRs, 4-week RWPS supplementation progressively decreased blood pressure, reaching 11.5% reduction at the end of the study (p < 0.001). RWPS consumption also increased the ferric reducing ability of plasma and attenuated the oxidation of plasma lipids and proteins, as evidenced by F-2-isoprostanes, malondialdehyde and protein carbonyl groups as oxidative stress biomarkers. Moreover, nitric oxide production (indirectly measured) was 1.5-fold higher in SHRs + RWPS than that in SHRs (p < 0.05). These beneficial effects were partly attributed to the ability of RWPS-derived bioactive compounds to modulate aortic gene expression, with eNOS, SOD2 and HO-1 over-expression, ACE down-regulation, and no changes in NOX4. In conclusion, this study suggests the potential of red wine pomace-derived seasonings to help in the management of hypertension.

• 出版日期2017-7-1