Multiclonal Expansion of Klebsiella pneumoniae Isolates Producing NDM-1 in Rio de Janeiro, Brazil

作者：Martins Aires Caio Augusto; Pereira Polyana Silva; Machado de Araujo Carlos Felipe; Gomes Chagas Thiago Pavoni; Ribeiro Oliveira Jane Cleide; Buonora Sibelle Nogueira; Albano Rodolpho Mattos; D%apos; Alincourt Carvalho Assef Ana Paula; Asensi Marise Dutra

来源：Antimicrobial Agents and Chemotherapy, 2017, 61(4): UNSP e01048-16.

DOI：10.1128/AAC.01048-16

<jats:title>ABSTRACT</jats:title>
<jats:p>
We characterized NDM-1-producing
<jats:named-content content-type="genus-species">Klebsiella</jats:named-content>
isolates from Rio de Janeiro, Brazil. PCR was applied for resistance and virulence determinants. The genetic context of
<jats:italic>bla</jats:italic>
<jats:sub>NDM</jats:sub>
was determined by S1 nuclease pulsed-field gel electrophoresis (PFGE) and hybridization. Genotyping was performed by PFGE and multilocus sequence typing (MLST). Most isolates carried multiple resistance genes and remained susceptible to amikacin, fosfomycin-trometamol, polymyxin B, and tigecycline. The spread of NDM-1-producing
<jats:named-content content-type="genus-species">Klebsiella pneumoniae</jats:named-content>
was not associated with clonal expansion and appears to be associated with Tn
<jats:italic>3000</jats:italic>
.
</jats:p>

• 出版日期2017-4