Analysis of LexA binding sites and transcriptomics in response to genotoxic stress in Leptospira interrogans

作者：Schons Fonseca Luciane; da Silva Josefa B; Milanez Juliana S; Domingos Renan H; Smith Janet L; Nakaya Helder I; Grossman Alan D; Ho Paulo L; da Costa Renata M A

来源：Nucleic Acids Research, 2016, 44(3): 1179-1191.

DOI：10.1093/nar/gkv1536

We determined the effects of DNA damage caused by ultraviolet radiation on gene expression in Leptospira interrogans using DNA microarrays. These data were integrated with DNA binding in vivo of LexA1, a regulator of the DNA damage response, assessed by chromatin immunoprecipitation and massively parallel DNA sequencing (ChIP-seq). In response to DNA damage, Leptospira induced expression of genes involved in DNA metabolism, in mobile genetic elements and defective prophages. The DNA repair genes involved in removal of photodamage (e.g. nucleotide excision repair uvrABC, recombinases recBCD and resolvases ruvABC) were not induced. Genes involved in various metabolic pathways were down regulated, including genes involved in cell growth, RNA metabolism and the tricarboxylic acid cycle. From ChIP-seq data, we observed 24 LexA1 binding sites located throughout chromosome 1 and one binding site in chromosome 2. Expression of many, but not all, genes near those sites was increased following DNA damage. Binding sites were found as far as 550 bp upstream from the start codon, or 1 kb into the coding sequence. Our findings indicate that there is a shift in gene expression following DNA damage that represses genes involved in cell growth and virulence, and induces genes involved in mutagenesis and recombination.

• 出版日期2016-2-18
• 单位MIT