科研之友
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摘要
The present study aimed to identify specific and upregulated proteins in virulent SS2 strain that might confer pathogenicity. We performed a comparative proteomic assay to analyze the difference in secretome between virulent SH-08 strain and avirulent SH-YH07 strain. Virulence of tested strains was determined using porcine and zebrafish models. The supernatant from bacterial cultures was collected at early exponential phase (OD600 = 0.4). Proteomic assay identified 52 proteins from the virulent strain supernatant and 29 proteins from the avirulent strain supernatant. Twenty-three proteins were found in the culture medium from both strains. Among these common proteins, eleven were upregulated by the virulent strain compared to the avirulent strain as shown by a label-free quantitative strategy. The mRNAs of all differentially secreted proteins were analyzed by real time PCR to determine if the transcription of these proteins was also different. Subsequently, the mRNA levels of differentiated proteins were further analyzed in two other virulent strains (HA9801, ZY05719) and one avirulent strain (T15). This investigation demonstrated major differences in the secretome between a pathogenic and a nonpathogenic strain of SS2.
- 出版日期2013-3
- 单位上海交通大学
