Analysis of calcium-induced effects on the conformation of fengycin

作者：Nasir Mehmet Nail; Laurent Pascal; Flore Christelle; Lins Laurence; Ongena Marc; Deleu Magali^*

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2013, 110: 450-457.

DOI：10.1016/j.saa.2013.03.063

• Conformational analysis
• Calcium
• Cyclic lipopeptide
• Bacillus subtilis
• Antimicrobial peptide

Fengycin is a natural lipopeptide with antifungal and eliciting properties and able to inhibit the activity of phospholipase A2. A combination of CD, FT-IR, NMR and fluorescence spectroscopic techniques was applied to elucidate its conformation in a membrane-mimicking environment and to investigate the effect of calcium ions on it. %26lt;br%26gt;We mainly observed that fengycin adopts a turn conformation. Our results showed that calcium ions are bound by the two charged glutamates. The calcium binding has an influence on the fengycin conformation and more particularly, on the environment of the tyrosine residues. The modulation of the fengycin conformation by the environmental conditions may influence its biological properties.

• 出版日期2013-6